A few days ago, Sichuan Government Procurement published online the Demonstration Opinions of the Expert Group of Imported Products of the Deyang Center for Disease Control and Prevention. The content shows that Deyang City Center for Disease Control and Prevention intends to purchase fluorescent quantitative PCR instrument, ultra-pure water device, pulsed field electrophoresis system, high-speed centrifuge, hydrogen generator, high-precision cross-temperature shaker, pipette 7 categories, a total of 16 Taiwan Instruments.
It is worth noting that the above seven types of instruments will all purchase imported products. Moreover, entrusted by the Deyang Center for Disease Control and Prevention, the expert group gave the following suggestions based on the understanding of the equipment industry and market such as fluorescent quantitative PCR instrument and the use of purchasers.
First, the fluorescence quantitative PCR instrument
The product to be purchased by the purchaser is mainly used for nucleic acid detection of epidemic influenza virus, hand-foot-mouth virus, intestinal pathogenic bacteria and other infectious diseases and food-borne pathogens. The instrument is required to detect the sample with short time and high sensitivity. Easy to operate and repeatable, the module can be upgraded when the sample size increases. At present, in the field of disease prevention and control, the detection of viral and bacterial nucleic acids (such as influenza virus, hand-foot-mouth virus, Shigella, Salmonella, etc.) using a real-time fluorescence quantitative PCR instrument can quickly identify the relevant pathogenic microorganism species.
At present, the domestic equipment can only rise and fall at a temperature of 2°C/sec or less. It takes more than 2h to complete an experiment and the detection time is longer. The domestic instrument has a temperature control accuracy of ±0.3°C, poor inter-hole reproducibility, and reproducibility of experimental results. Not high; domestic instruments only have 1-3 detection channels, can only detect 3 genes at the same time, the detection efficiency is low; do not have the upgrade function, can not meet the requirements of the purchaser detection.
The temperature of the imported products is fast, the inter-hole repeatability is good, and the repeatability of the experimental results is ensured; the detection channels of imported instruments are high, up to 4 to 5, and 3 to 5 genes can be detected at a time, improving the detection efficiency; Upgrade to a faster module to meet the rapid demand; visible, domestic equipment can not meet the needs of the purchaser, the buyer's desire to purchase imported products is necessary and reasonable.
Therefore, it is recommended that you agree to purchase imported products in order to meet your business needs.
Second, ultra pure water device
The pure water/ultra-pure water system uses tap water as the influent, and the pretreatment-RO membrane-EDI module-ultraviolet lamp-purification and purification process. At the same time, continuous production of analytical grade pure water (Grade II water) for glassware, final rinse, chemical/biochemical reagent preparation, analysis reagents, and pharmaceutical formulation, dilution, etc., and for cell culture, Q-PCR, peptide mapping, and other organisms Ultrapure water (Grade I water) required for ultrapure water and high-precision analytical equipment (AAS, IC, HPLC, LC, GC-MS, etc.) for engineering experiments. At present, due to the immature purification technology, domestic water purifiers cause large fluctuations in water quality, which has a great impact on the analysis results and the life of the analytical instruments. The core purification technology of imported ultrapure water machines is currently not available for domestic water machines and cannot meet the buyer's experimental analysis requirements. details as follows:
1. Accuracy of Conductivity Meter: Conductivity meter is used to measure the conductivity of ultrapure water, which directly reflects the content of various charged ions in water. The higher the ion content, the worse the water quality. Atomic absorption, ICP, and ion chromatography require extremely low levels of ions in water. Ultrapure water is required to have a conductivity of 0.055 μS/cm (18.2 MΩ·cm@25°C). The key to accurate measurement is the accuracy of the conductivity meter. The electrode constant represents the accuracy of the conductivity meter. The imported water machine has an on-line conductivity meter with an electrode constant of 0.01-0.1 cm-1 and automatic temperature compensation. The domestic water machine is 0.1-0.5 cm-1 and does not have temperature compensation.
2. TOC (total organic carbon) monitor: The level of TOC in ultrapure water affects the analysis of instruments such as HPLC and LC-MS. High concentration of TOC will cause pollution to the instrument, interfere with baseline and signal background noise and cause miscellaneous Peaks affect the degree of separation and are difficult to quantify. The imported water machine built-in TOC monitor can monitor the TOC content in the water. Detection range 1-999ppb, detection accuracy ± 1ppb. With independent quartz detection chamber, independent 185nm UV lamp. The domestic water machine currently does not have an independent TOC monitor. The main difference is the conductivity. The TOC value cannot pass the measurement certification.
3. Anti-scaling EDI technology (continuous current deionization technology): EDI technology is a kind of purification technology that uses weak current to regenerate resin, and is mainly used to remove trace amounts of charged ions and charged organic substances in water. Produce secondary water of 5-15MΩ·cm @25°C without consumables and the water quality is more stable. At present, domestic water machines do not have this technology and still rely on traditional RO+DI ​​technology.
4. The imported water purification column uses artificial silver-plated activated carbon and nuclear-grade ion exchange resin, which has high ion removal rate and greater water treatment volume. Artificial silver-plated activated carbon can effectively remove organic matter and residual chlorine in water, and the adsorption capacity of the same amount of artificial silver-plated activated carbon is two to three times that of natural activated carbon. Due to cost constraints, domestic water machines currently use common ion exchange resins and natural activated carbon as the packing for the purification column.
5, a variety of terminal filter options: imported water function for different applications with different terminal filters, can remove ultra-pure water particles, bacteria, RNase, DNA enzymes, heat, trace organic matter, endocrine disruptors Wait. Domestic water machines can only provide end filters that remove bacteria and particles.
6. RFID electronic identification technology: RFID technology can record the installation time, usage time, and the amount of water in a timely manner to facilitate laboratory certifications such as CNAS. Domestic water machines do not have this technology, nor can they pass CNAS certification.
Therefore, it is recommended that you agree to purchase imported products in order to meet your business needs.
Third, the pulse field electrophoresis system
The pulsed field electrophoresis system is mainly used for traceability of pathogenic factors and etiological foods of foodborne diseases. The system needs to separate 100bp-10Mb DNA fragments; pulse angle range: 0-360. , can achieve 0.5 per step. The adjustment; Based on the CHEF technology (clamp shimming) pulse control; conversion time gradient linear, convex and concave three, the conversion time 50 msec -18 hr; real-time monitoring and regulation of the electrode potential; up to 8 The program module settings can be executed automatically and continuously; at the same time, the analysis software can be matched with the PulseNet data network to analyze and compare the results. As an important tool for the detection of foodborne pathogenic microorganisms, the pulsed field gel electrophoresis system has been widely used in various disease control centers, inspection and quarantine agencies, food safety and public health fields, and large hospitals. The system uses the international PulseNet network database very well and serves as the gold standard for traceable pathogens and molecular typing in the PulseNet network. At present, the accuracy of temperature control of domestic products is unstable and the variable angle is too small to achieve a good separation of similar DNA fragments; it also does not have the function of connecting with PulseNet network, and it is impossible to conduct experimental data analysis and comparison, and cannot satisfy procurement. Demand for traceability analysis of pathogenic bacteria in humans. So apply for purchasing imported equipment.
Fourth, high-speed centrifuge
It is mainly used for centrifugation of cryotubes. The maximum speed of the imported centrifuge can reach more than 17000rpm, and the maximum centrifugal force exceeds 30000xg, which can meet the laboratory's sample processing requirements. At the same time, the imported centrifuge has a SOFT soft brake function to protect valuable samples such as blood and prevent the sample from resuspending. Its automatic rotor identification function, automatic rotor imbalance recognition, makes centrifuging safer and protects experimental operators. Its fixed-speed timing function achieves a predetermined speed and then starts timing. This improves the reproducibility and temperature of the experiment. The imported centrifuge has a programmable function. It can store 50 frequently-used programs and 5 shortcut program keys to quickly run common programs. . Domestic equipment can not meet the purchaser's handling of special products. So purchase imported products.
Fifth, hydrogen generator:
1. The purity of the imported hydrogen generator is ≥99.9995%, and the domestic product can only reach 99.995%, which cannot meet the demand of the purchaser for high-precision analysis.
2. The imported hydrogen generator adopts the LCD panel touch panel design of the microcomputer control system, which can be read and consulted for various operating parameters and conditions to ensure reliable and stable operation. Domestic products do not have this function and cannot guarantee the quality of the gas and cause damage to the analytical instrument.
3. When the imported hydrogen generator operates abnormally, the system automatically diagnoses the cause of the failure and displays different color interfaces and text information. Domestic products do not have this alarm function.
4. The imported hydrogen generator adopts internal and external double tank design (no additional configuration or separate placement) to ensure the quality of water and electrolyzer performance. The domestic product adopts a single tank design and cannot guarantee the quality of water and electrolyzer performance.
5, imported hydrogen generator chassis using high-strength stainless steel, with fire and explosion protection, domestic equipment without this function
It is recommended to purchase imported products.
Six, high precision horizontal temperature shaker
1. Temperature range of imported constant temperature shaker: 4°C-100°C above room temperature, oscillation speed: 300～1,500 rpm, temperature control accuracy: ±0.5°C, temperature stability: 0.5°C, temperature uniformity: 0.5°C, maximum heating Rate: 11 °C/min; domestic instruments can not meet the buyer's experimental requirements at the same time;
2. The imported brands can maintain good performance in terms of temperature stability and homogeneity. The homogeneity and stability of domestic instruments are relatively lacking. For example, the accuracy of temperature control is usually 1°C, and the maximum heating rate is 5°C/minute. Can not meet the experimental needs.
It is recommended to purchase imported products.
Seven, pipette
At present, there are few domestically produced products, which have poor accuracy and poor repeatability, and cannot meet the needs of purchasers. Therefore, the purchaser's desire to purchase imported products is reasonable. details as follows:
1. Electro-assisted pipettes: It is mainly used for the removal of liquids. The quality of imported products is stable, and the battery has a long service life. It can carry out 2000 pipetting operations at one time, which saves working time. Imported products have an internal balance system designed to prevent liquids from entering the suction device and cause corrosion. When the liquid is drawn faster, the domestic product can easily enter the cavity and damage the machine, greatly affecting the service life. The imported product has an intuitive speed control button, which can control the speed of liquid suction by finger pressure, and domestic products do not have this function. So purchase imported products.
2, bottle mouth dispenser is mainly used for the removal of conventional liquid, corrosive, volatile liquids and packaging. The imported product has a wide range of liquid separation and is available in 0.5ml-100ml volumes, and can be finely adjusted according to the liquid viscosity. Domestic equipment does not have this function. The imported product has a safety valve design that can be dispensed in a highly repetitive manner without wasting reagents. Domestic equipment does not have this design. The imported products have a clean piston design to prevent the liquid from crystallizing. The domestic equipment has not yet possessed this function, so the imported products are purchased.
3. Manual single-channel pipettes: mainly used to accurately remove liquids. The main technical indicators and performance descriptions of imported products and similar domestic products: Can be sterilized in whole; there are multiple ranges to choose from, 0.1-2.5μl, 0.5-10μl, 2-20μl, 10-100μl, 20-200μl, 100-1000μl, domestic pipette minimum range can not reach 0.1μl. At the same time, the inaccuracy of imported products is low, which can reach ±0.2%. The inaccuracy of domestic products can only reach 0.5%. Comparing the advantages and disadvantages of imported products with similar domestic products in terms of technical indicators and performance: Compared with domestic products, imported products are highly accurate; they have four digits of enlarged digital display and density adjustment functions, and they are lightweight, robust, and capable of handling due to the use of advanced materials. Sterilization;Progressive resistance is small, operation feel is good.
So purchase imported products.
And none of the above-mentioned equipment was listed in the "China's Catalogue of Import Prohibited Import Restrictions" and "Catalogue of Imported Electromechanical Products".
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