The background of the dual-beam UV detector is a UV detector in liquid chromatography, which is used to monitor biochemistry, molecular biology, pharmaceutical, food and other industries in the column. Chromatography is essential equipment for separation and analysis. Most of the products currently in the market are “nucleic acid protein detectorsâ€. This instrument is basically a product transferred by Biochemistry in the 1970s, given the technical level, market components, etc. Restricted, so although the trial system was successful, there are still many problems, such as baseline drift, shift zero, etc. For this reason, although there are more than a dozen manufacturers on the market, they are almost the same product, the same face, the same performance, Unable to meet the needs of users. Imported instruments such as Pharmacia are expensive. The domestic nucleic acid protein detectors are far behind the market, and many of the urgently needed improvements have rarely been researched and remodeled by manufacturers. In the past 20 years, except for a slight improvement, the internal structure has remained almost unchanged.
Inadequacies:
1. The light source of the instrument, because the mercury protein lamp is used in the nucleic acid protein detection instrument, its specific line is 253.7nm, and the wavelength for detecting nucleic acid in the biochemical industry is 260nm, and the problem is not in the sample with high detection concentration. Large, but in the case of a small amount of valuable samples and the concentration is relatively thin, the resulting deviation is large.
2. In the photoelectric conversion, the nucleic acid protein detector uses a photomultiplier tube. We know that the photomultiplier tube is large in size and covers a large area, and also requires high-voltage power supply support. The stability of the high-voltage power supply directly affects the stability of the entire instrument. Whether it is very important or not, plus the undercurrent of photomultiplier, uncertainty with temperature, etc., is one of the fundamental reasons why the instrument is not easy to do. Now the imported instruments on the market basically use photodiodes to make converters. Small size (only one transistor), stable performance, small undercurrent, such advanced technology nucleic acid protein detector is abandoned.
3. The nucleic acid protein detector on the market now seems to have a wavelength of 254 nm and 280 nm, which can be measured substantially at the time of measuring the nucleic acid at 254 nm (the true nucleic acid measurement is 260 nm), but it is somewhat far-fetched when the protein is measured at 280 nm. Because their energy is very weak at this time, the imported instruments use the mercury lamp as the light source to measure the protein. Their 280nm wavelength is obtained by fluorescence to convert 254nm to 280nm by fluorescence, and then 280nm wavelength to obtain 280nm wavelength to detect protein. However, in the domestic nucleic acid protein detection instrument, because there is no such technology (the phosphor is not toxic, and there is no such technology), so the process can be omitted, and only the 280 nm filter can be used to obtain the wavelength of 280 nm. As a result, since the characteristic line of the mercury lamp is 254 nm, the wavelength of 280 nm is an extension of the line, and the light intensity is almost 1/10 of that of 254 nm, so although the 280 nm filter is used, the energy of 254 nm is too strong, It can still pass through the filter to enter the measurement system. The result is a common absorption peak of 254nm and 280nm wavelength. Therefore, this kind of instrument can be used as an educational tool. In the research field, it is very very in the pharmaceutical industry. Adverse.
4. There is a problem in the design of the circuit for the nucleic acid protein detector on the market. For example, when the sensitivity is changed without a sample, the baseline reflected by the recorder will change greatly, which is very troublesome for the operator if the peak is found during the monitoring process. If the shape is too large or too small, if you want to change the sensitivity to get the appropriate peak, the peak will be affected due to the change of the baseline reference point, and the result will be inaccurate. When the sensitivity is >1 OD, the instrument zero point and the recorder zero point are greatly deviated. can not work.
5. There is also a UV detection instrument on the market that uses elemental lamps as the light source. Although the spectrum length of the lamp is better than that of the mercury lamp, the element lamp has a short life span of 2000 hours, which is not suitable as a long time. Monitoring, frequent replacement of lamps is costly.
In addition, the nucleic acid protein detectors on the market are basically sensitive shifts. Not only the baseline of the recorder changes, but also the reading of the meter changes. In fact, the sensitivity shift does not change the concentration of a sample, and the recorder is changed. Peak size, concentration reading is constant.
In view of the problems in the nucleic acid protein detectors on the market, and their negative consequences for researchers, the pharmaceutical industry and other industries, and to fill the domestic gap, it was decided to try the difficult double-beam UV detector. Through the two-year research and development of biochemical professional technicians, the new-generation UV-DETECTOR III double-beam UV detector has been introduced to the market. It has been proved by Lv and other imported instruments through the application of major universities, scientific research institutes and biopharmaceuticals. The same effect.
Second, the double-beam UV detector and other UV detectors 1. The source of the ultraviolet light source dual-beam UV detector is an electrodeless discharge lamp, which is blank in China. It is only produced by the Swedish LKB company. Through the investigation of documents by professionals, the continuous trial production will ultimately lead to success.
2. Circuit design The photo-electric diode of the photoelectric converter of the double-beam UV detector is used to keep up with the requirements of the times, eliminating the high voltage and high voltage and affecting the instability of the instrument. The line is in the form of a double beam, one for the sample beam and the other for the reference beam. After the reference beam is converted to a voltage, it is used to generate feedback to suppress the change of the source with temperature, so that the whole machine is stable and does not resemble a single beam. That way, due to the influence of temperature, etc., it drifts slowly.
3. The temperature control lamp room is controlled by constant temperature. As we all know, the general light source will change with temperature. The constant temperature mode not only stabilizes the light source, but also prolongs the life of the lamp, and is also suitable for long-term use of the instrument in the cold room.
The electrodeless discharge lamp is used, the small size is only the size of the finger, and the power supply part of the starting light source is excited by microwave. The whole excitation light source plate is only palm size, the structure is simple, the power consumption is <3W, the lamp has a long service life, theoretically 100,000 hours, on the instruction manual. Conservative writing for 20,000 hours, long-term continuous use without shutting down, fully suitable for long-term surveillance in biochemical fields,
Dual beam UV detector features:
1. The instrument has a short settling time and is stable enough within half an hour after starting up.
2, the instrument shell design anti-corrosion, anti-rust, beautiful, lightweight, the market has not seen similar products.
3, the circuit design is advanced and reasonable, in addition to the use of feedback and other technologies, when the instrument changes sensitivity, the zero point baseline on the recorder remains basically unchanged, and the readings on the panel table do not change with sensitivity changes, the experimental results are correct and reliable.
4. Because the light source uses an electrodeless discharge lamp, the intensity of each wavelength is 214nm, 230nm, 260nm, 280nm, 214nm, 340nm, etc., and the wavelength is taken out by the filter, the monochromaticity is good, and it will not bring to the operator, the researcher, etc. Inter-wavelength interdiffusion effect.
Compared with imported LKB instruments, we have adopted their advanced technology, but have improved them. Like electrodeless discharge lamps, they use 2 filters and 2 lamps for 260nm and 280nm, and we only need one. The lamp can get 5 wavelengths, which can adapt to different people's needs and has a wider application range. Imported instruments do not have a panel meter, and we use panel meters to be more intuitive, and to obtain monitoring sample information from the meter at any time, even if the instrument is not connected to the recorder, the dual-beam UV detector is more stable than imported instruments, especially at high Within the sensitivity area.
Inadequacies:
1. The light source of the instrument, because the mercury protein lamp is used in the nucleic acid protein detection instrument, its specific line is 253.7nm, and the wavelength for detecting nucleic acid in the biochemical industry is 260nm, and the problem is not in the sample with high detection concentration. Large, but in the case of a small amount of valuable samples and the concentration is relatively thin, the resulting deviation is large.
2. In the photoelectric conversion, the nucleic acid protein detector uses a photomultiplier tube. We know that the photomultiplier tube is large in size and covers a large area, and also requires high-voltage power supply support. The stability of the high-voltage power supply directly affects the stability of the entire instrument. Whether it is very important or not, plus the undercurrent of photomultiplier, uncertainty with temperature, etc., is one of the fundamental reasons why the instrument is not easy to do. Now the imported instruments on the market basically use photodiodes to make converters. Small size (only one transistor), stable performance, small undercurrent, such advanced technology nucleic acid protein detector is abandoned.
3. The nucleic acid protein detector on the market now seems to have a wavelength of 254 nm and 280 nm, which can be measured substantially at the time of measuring the nucleic acid at 254 nm (the true nucleic acid measurement is 260 nm), but it is somewhat far-fetched when the protein is measured at 280 nm. Because their energy is very weak at this time, the imported instruments use the mercury lamp as the light source to measure the protein. Their 280nm wavelength is obtained by fluorescence to convert 254nm to 280nm by fluorescence, and then 280nm wavelength to obtain 280nm wavelength to detect protein. However, in the domestic nucleic acid protein detection instrument, because there is no such technology (the phosphor is not toxic, and there is no such technology), so the process can be omitted, and only the 280 nm filter can be used to obtain the wavelength of 280 nm. As a result, since the characteristic line of the mercury lamp is 254 nm, the wavelength of 280 nm is an extension of the line, and the light intensity is almost 1/10 of that of 254 nm, so although the 280 nm filter is used, the energy of 254 nm is too strong, It can still pass through the filter to enter the measurement system. The result is a common absorption peak of 254nm and 280nm wavelength. Therefore, this kind of instrument can be used as an educational tool. In the research field, it is very very in the pharmaceutical industry. Adverse.
4. There is a problem in the design of the circuit for the nucleic acid protein detector on the market. For example, when the sensitivity is changed without a sample, the baseline reflected by the recorder will change greatly, which is very troublesome for the operator if the peak is found during the monitoring process. If the shape is too large or too small, if you want to change the sensitivity to get the appropriate peak, the peak will be affected due to the change of the baseline reference point, and the result will be inaccurate. When the sensitivity is >1 OD, the instrument zero point and the recorder zero point are greatly deviated. can not work.
5. There is also a UV detection instrument on the market that uses elemental lamps as the light source. Although the spectrum length of the lamp is better than that of the mercury lamp, the element lamp has a short life span of 2000 hours, which is not suitable as a long time. Monitoring, frequent replacement of lamps is costly.
In addition, the nucleic acid protein detectors on the market are basically sensitive shifts. Not only the baseline of the recorder changes, but also the reading of the meter changes. In fact, the sensitivity shift does not change the concentration of a sample, and the recorder is changed. Peak size, concentration reading is constant.
In view of the problems in the nucleic acid protein detectors on the market, and their negative consequences for researchers, the pharmaceutical industry and other industries, and to fill the domestic gap, it was decided to try the difficult double-beam UV detector. Through the two-year research and development of biochemical professional technicians, the new-generation UV-DETECTOR III double-beam UV detector has been introduced to the market. It has been proved by Lv and other imported instruments through the application of major universities, scientific research institutes and biopharmaceuticals. The same effect.
Second, the double-beam UV detector and other UV detectors 1. The source of the ultraviolet light source dual-beam UV detector is an electrodeless discharge lamp, which is blank in China. It is only produced by the Swedish LKB company. Through the investigation of documents by professionals, the continuous trial production will ultimately lead to success.
2. Circuit design The photo-electric diode of the photoelectric converter of the double-beam UV detector is used to keep up with the requirements of the times, eliminating the high voltage and high voltage and affecting the instability of the instrument. The line is in the form of a double beam, one for the sample beam and the other for the reference beam. After the reference beam is converted to a voltage, it is used to generate feedback to suppress the change of the source with temperature, so that the whole machine is stable and does not resemble a single beam. That way, due to the influence of temperature, etc., it drifts slowly.
3. The temperature control lamp room is controlled by constant temperature. As we all know, the general light source will change with temperature. The constant temperature mode not only stabilizes the light source, but also prolongs the life of the lamp, and is also suitable for long-term use of the instrument in the cold room.
The electrodeless discharge lamp is used, the small size is only the size of the finger, and the power supply part of the starting light source is excited by microwave. The whole excitation light source plate is only palm size, the structure is simple, the power consumption is <3W, the lamp has a long service life, theoretically 100,000 hours, on the instruction manual. Conservative writing for 20,000 hours, long-term continuous use without shutting down, fully suitable for long-term surveillance in biochemical fields,
Dual beam UV detector features:
1. The instrument has a short settling time and is stable enough within half an hour after starting up.
2, the instrument shell design anti-corrosion, anti-rust, beautiful, lightweight, the market has not seen similar products.
3, the circuit design is advanced and reasonable, in addition to the use of feedback and other technologies, when the instrument changes sensitivity, the zero point baseline on the recorder remains basically unchanged, and the readings on the panel table do not change with sensitivity changes, the experimental results are correct and reliable.
4. Because the light source uses an electrodeless discharge lamp, the intensity of each wavelength is 214nm, 230nm, 260nm, 280nm, 214nm, 340nm, etc., and the wavelength is taken out by the filter, the monochromaticity is good, and it will not bring to the operator, the researcher, etc. Inter-wavelength interdiffusion effect.
Compared with imported LKB instruments, we have adopted their advanced technology, but have improved them. Like electrodeless discharge lamps, they use 2 filters and 2 lamps for 260nm and 280nm, and we only need one. The lamp can get 5 wavelengths, which can adapt to different people's needs and has a wider application range. Imported instruments do not have a panel meter, and we use panel meters to be more intuitive, and to obtain monitoring sample information from the meter at any time, even if the instrument is not connected to the recorder, the dual-beam UV detector is more stable than imported instruments, especially at high Within the sensitivity area.
Tie- bared efficient aerosol transforming mechanism
Multi- core pulling modes for choosing
Self- developed efficient, energy- saving servo system
Low pressure and high pressure patent for options, suitable for different products
Efficient plasticized screw with high length- to- diameter ration( Design by Germany)
Specified Injection Moulding process for Bi- section pipes
Patented center clamping structure
Double- cylinders for moving, single cylinder for injection
Related Products:Plastic Water Pipe Making Machine,Pipe Making Machine.
EKH Large Volume Injection Moulding Machine
Large Volume Injection Moulding Machine, pipe fitting injection moulding machine, Pipe Fitting Injection Machine,Plastic Water Pipe Making Machine,Pipe Making Machine
Ningbo Shuangma Machinery Industry Co., Ltd , https://www.bolemachinery.com