Chlorophyll in plant leaves plays an important role in the process of photosynthesis, and its content is an important indicator for evaluating the physiological status of plants. By detecting the concentration of chlorophyll, it is possible to accurately determine the growth of plants. To measure chlorophyll using traditional spectrophotometry, the sample needs to be extracted with acetone, and then the sample is measured using a spectrophotometer. Although the traditional spectral broad method has high measurement accuracy, this method of detection damages plant leaves, takes a long time and is not convenient for continuous measurement, and can not meet the requirements of fast and non-destructive testing in the field. Therefore, for the current situation that China lacks high-precision, low-cost chlorophyll rapid detection equipment for crop leaves in the field, research and development of a portable chlorophyll detection device based on single-chip microcomputer. The chlorophyll meter was successfully developed under this condition.
There are two main methods for measuring leaf chlorophyll content in living plants: reflection and transmission. The reflectance method can be used to determine the condition of a patch of vegetation and can also be used to determine single leaves. The instruments used in the reflection method are complex, and the reflection of light by the leaves is related to the surface condition of the leaves, and also related to the distribution of chlorophyll in the leaves. Therefore, the error is relatively large, and the chlorophyll determination in the sea area is often used. When the transmission method is used to measure the chlorophyll content in the leaves, the effects of other pigments in the leaves, the shape of the leaves, and the measurement position and time of the leaf on the measurement are taken into account. Generally, the dual wavelength method is used to eliminate this effect.
The chlorophyll meter is designed to facilitate the storage of data, and an SD card module has been designed. At the same time, in order to reflect the location geographical information of the tested blade in real time, a GPS module is designed as a peripheral device, and an RS232 module is designed as a communication port. The instrument overcomes the insufficiency of data storage in other similar products, adds GPS function, realizes measurement of location information of measurement sites, and has functions of saving time, date, ambient temperature, humidity, and other information. By comparison with the Japanese SPAD-502 instrument, the correctness and realizability of the instrument for the non-destructive detection of chlorophyll content by the instrument are proved. The instrument is easy to carry and suitable for field testing. It is convenient for promotion in our country.
There are two main methods for measuring leaf chlorophyll content in living plants: reflection and transmission. The reflectance method can be used to determine the condition of a patch of vegetation and can also be used to determine single leaves. The instruments used in the reflection method are complex, and the reflection of light by the leaves is related to the surface condition of the leaves, and also related to the distribution of chlorophyll in the leaves. Therefore, the error is relatively large, and the chlorophyll determination in the sea area is often used. When the transmission method is used to measure the chlorophyll content in the leaves, the effects of other pigments in the leaves, the shape of the leaves, and the measurement position and time of the leaf on the measurement are taken into account. Generally, the dual wavelength method is used to eliminate this effect.
The chlorophyll meter is designed to facilitate the storage of data, and an SD card module has been designed. At the same time, in order to reflect the location geographical information of the tested blade in real time, a GPS module is designed as a peripheral device, and an RS232 module is designed as a communication port. The instrument overcomes the insufficiency of data storage in other similar products, adds GPS function, realizes measurement of location information of measurement sites, and has functions of saving time, date, ambient temperature, humidity, and other information. By comparison with the Japanese SPAD-502 instrument, the correctness and realizability of the instrument for the non-destructive detection of chlorophyll content by the instrument are proved. The instrument is easy to carry and suitable for field testing. It is convenient for promotion in our country.
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