Wheat grain establishment is a complex physiological process involving the function and mutual assistance of the source bank and is controlled by endogenous hormones. In order to clarify the actual regulation of sucrose on the number of grains per spike, it is necessary to observe changes in the number of grains per ear by changing the supply of carbon material to the wheat ears through a light incubator. However, under field conditions, it is very difficult to study the regulation of sucrose on grains per ear, because the supply of sucrose to wheat ears cannot be controlled effectively.
After retrieving the plants, remove the leaves, retain the flag leaves and the leaf sheaths of each leaf, cut from the middle of the inverted 2 leaf internodes, sterilize the isolated stems and ears with 0.1% mercuric chloride solution for 10 minutes, and then wash 3 times with sterile water. And cut about 2cm in the lower end of the internode in sterile water, and immediately insert the light incubator into the culture bottle. The flasks were filled with 250 ml wide mouth vials containing 150 ml of basic medium. Three spikes were inserted into each vial and the replicates were repeated four times. The mouth of the flask was wrapped with cotton plugs and the stems and stems were fixed. Basic culture components include MS inorganic components, sucrose concentration determined by the test. Cultured in a light incubator with a daily light time of 14 to 16 hours (40 W/m2 fluorescent lamp). Temperature 20 ~ 25 °C.
In vitro spike culture process, n, can be controlled by adjusting the sucrose concentration of the medium by light incubator. From 13 days before flowering to 1 day before flowering, the seed setting rate of Liu Yisui with different concentrations of sucrose had different effects. With the increase of sucrose concentration, the number of grains per spike increased, but the concentration of sucrose exceeded 4%, and the seed setting rate did not increase. cut back. It can be seen from the light incubator that at a concentration of 0.0%-0.5% sucrose, there is still a certain amount of cattle growth among the upper bamboos, but the growth is slow and heading cannot be achieved.
At different periods before flowering, under the control of different sucrose supply, the effect of sucrose on grains per spike was investigated. The light incubator test results showed that the development of florets before flowering was constrained by sucrose supply. When sucrose concentration was less than 4%, increasing the sucrose concentration could increase the seed setting rate of differentiated florets. Over 4% sucrose concentration, the number of florets increased and the seed setting rate decreased. However, in-depth study of the process of grain formation, 1, the assimilation supply is of great significance for uncovering the regulation mechanism of grain formation.
After retrieving the plants, remove the leaves, retain the flag leaves and the leaf sheaths of each leaf, cut from the middle of the inverted 2 leaf internodes, sterilize the isolated stems and ears with 0.1% mercuric chloride solution for 10 minutes, and then wash 3 times with sterile water. And cut about 2cm in the lower end of the internode in sterile water, and immediately insert the light incubator into the culture bottle. The flasks were filled with 250 ml wide mouth vials containing 150 ml of basic medium. Three spikes were inserted into each vial and the replicates were repeated four times. The mouth of the flask was wrapped with cotton plugs and the stems and stems were fixed. Basic culture components include MS inorganic components, sucrose concentration determined by the test. Cultured in a light incubator with a daily light time of 14 to 16 hours (40 W/m2 fluorescent lamp). Temperature 20 ~ 25 °C.
In vitro spike culture process, n, can be controlled by adjusting the sucrose concentration of the medium by light incubator. From 13 days before flowering to 1 day before flowering, the seed setting rate of Liu Yisui with different concentrations of sucrose had different effects. With the increase of sucrose concentration, the number of grains per spike increased, but the concentration of sucrose exceeded 4%, and the seed setting rate did not increase. cut back. It can be seen from the light incubator that at a concentration of 0.0%-0.5% sucrose, there is still a certain amount of cattle growth among the upper bamboos, but the growth is slow and heading cannot be achieved.
At different periods before flowering, under the control of different sucrose supply, the effect of sucrose on grains per spike was investigated. The light incubator test results showed that the development of florets before flowering was constrained by sucrose supply. When sucrose concentration was less than 4%, increasing the sucrose concentration could increase the seed setting rate of differentiated florets. Over 4% sucrose concentration, the number of florets increased and the seed setting rate decreased. However, in-depth study of the process of grain formation, 1, the assimilation supply is of great significance for uncovering the regulation mechanism of grain formation.
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