Dongtian Zhongbiao Guangzhou University of Traditional Chinese Medicine 3 High-speed Countercurrent Chromatography Instrument Project

This month, Tongtian Biological, as the world's leading supplier of high-speed countercurrent chromatographs, successfully won the bid for 3 high-speed countercurrent chromatograph projects at Guangzhou University of Chinese Medicine.

Successful bidder:

Analytical high-speed countercurrent chromatograph + semi-preparative high-speed countercurrent chromatograph

Background technology

High-speed counter current chromatography (HSCCC) is a continuous high-efficiency liquid-liquid chromatographic separation technology developed in the 1980s. It does not use any solid support or carrier. It uses a two-phase solvent system to establish a special unidirectional fluid dynamic equilibrium in a spiral tube rotating at a high speed. When one phase is used as the stationary phase and the other phase is used as the mobile phase, it can be retained during continuous elution. A lot of stationary phase.

Since the solid support is not required, the separation of the substance is realized by the difference in the partition coefficient between the two phases, thereby avoiding the loss, inactivation, and degeneration of the sample due to irreversible adsorption, and not only recovering the sample completely but also recovering the sample. The sample more reflects its original characteristics and is particularly suitable for the separation of natural biologically active ingredients. Moreover, due to the sufficient contact between the separated substance and the liquid stationary phase, the preparation amount of the sample is greatly improved, and it is an ideal preparation and separation method.

Compared with the traditional solid-liquid column chromatography technology, it has the advantages of wide application scope, flexible operation, high efficiency, rapidity, large amount of preparation, and low cost. At present, HSCCC technology is developing into a new type of separation and purification technology that has attracted much attention. It has been widely used in biomedicine, natural products, food and cosmetics, and is particularly considered as an effective new separation technology in the natural product industry. Suitable for the separation and purification of small and medium-sized molecules.

China is the first country to develop countercurrent chromatography after the United States and Japan. Russia, France, the United Kingdom, and Switzerland have also carried out this study. The US FDA and the World Health Organization (WHO) have cited this technology as a separation test for antibiotic components. Since the 1990s, high-speed countercurrent chromatography has been widely used in the separation preparation and analysis of natural pharmaceutical ingredients.